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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | FC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rat IgG2b |
| Clone No. | M5/114 |
| Immunogen | Activated C57BL/6 mouse spleen cells |
| Target | MHC Class II |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Separation of murine MHC Class II positive splenocytes (red-filled) from MHC Class II negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/ml, Streptavidin APC).
Flow cytometry surface staining pattern of murine splenocyte suspension using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/ml, Streptavidin APC).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
MHC Class II Antibody (Biotin) (orb738511)
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