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RPS6 (Phospho-S235) Rabbit Polyclonal Antibody
SKU: orb223999
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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IF/ICC: 1:100-1:500 |
| Reactivity | Bovine, Gallus, Human, Monkey, Mouse, Rat, Sheep, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S235 of human RPS6 protein. The exact sequence is proprietary. |
| Target | RPS6 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−40S ribosomal protein S6; Phosphoprotein NP33
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Western blot analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 32 kD)
Immunofluorescent analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
RPS6 (Phospho-S235) Rabbit Polyclonal Antibody (orb223999)
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