SOX9 (Phospho-S181) Rabbit Polyclonal Antibody

Rating: 5
Author: Zhou Yongjian

SKU: orb214601

This product can be custom made

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Description

The SOX9 (Phospho-S181) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S181 of human SOX9 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC, WB
Dilution Range	WB: 1-500-1-1000
Reactivity	Canine, Human, Mouse, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S181 of human SOX9 protein. The exact sequence is proprietary.
Target	SOX9
Molecular Weight	56 kDa
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Transcription factor SOX-9

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SOX9 (Phospho-S181) Rabbit Polyclonal Antibody

Western blot analysis of SOX9 (Phospho-S181) expression in DLD (A), HEK293T (B), A375 (C) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 56 kD)

Immunofluorescent analysis of SOX9 (Phospho-S181) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of paraffin-embedded mouse brain using SOX9 (Phospho-S181) Rabbit Polyclonal Antibody orb214601 with dilution at 1:100.

Quick Database Links

Gene Symbol

SOX9

UniProt

P48436, Q04887

NCBI Gene ID

6662, 20682

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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