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SPARC Antibody
SKU: orb2651056
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Reactivity | Human, Mouse, Rat, Virus, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | SPARC |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
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Western blot analysis of SPARC expression in A549 (A), mouse kidney (B), mouse brain (C), rat brain (D) whole cell lysates.
Immunohistochemical analysis of SPARC staining in mouse muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
SPARC Antibody (orb2651056)
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