Anti-SUV420H2 [RAB-C409]

SKU: orb1089945

Description

Rabbit polyclonal antibody to SUV420H2

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	ChIP, ELISA
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	Human IgG
Clone No.	RAB-C409
Immunogen	This antibody was obtained by recombinant antibody (rAb) phage display recognizing SUV420H2 protein under non-denaturing conditions.
Target	SUV420H2
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Suv4-20h2; Suppressor of variegation 4-20 homolog 2; Histone-lysine N-methyltransferase KMT5C; Lysine N-methyltransferase 5C; Lysine-specific methyltransferase 5C; Su(var)4-20 homolog 2; [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B; [histone H4]-lysine20 N-methyltransferase KMT5B; 2.1.1.362; 2.1.1.361; anti-SUV420H2-RAB-C409; 48G1_11

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Flow cytometry using the anti-SUV420H2 antibody RAB-C409. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RAB-C409 (orb1089945, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-C409 (orb1089945) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom orb1089945, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

SUV420H2

UniProt

Q86Y97

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ChIP

Chromatin Immunoprecipitation

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