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TAU (Phospho-T498) Rabbit Polyclonal Antibody
SKU: orb216149
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Description
Research Area
Alzheimer's, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T498 of human TAU protein. The exact sequence is proprietary. |
| Target | MAPT |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MAPTL; MTBT1; TAU; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau
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Western blot analysis of TAU (Phospho-T498) expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 50 kD)
Immunofluorescent analysis of TAU (Phospho-T498) staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
TAU (Phospho-T498) Rabbit Polyclonal Antibody (orb216149)
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