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Description
Research Area
Cancer, DNA Damage, Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:1000, IF/ICC: 1:100-1:500 |
| Reactivity | Bovine, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Thymine DNA Glycosylase. The exact sequence is proprietary. |
| Target | TDG |
| Molecular Weight | 46 kDa |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; hTDG
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Immunofluorescent analysis of Thymine DNA Glycosylase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of paraffin-embedded mouse testis using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded mouse thymus using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:100.
Western blot analysis of mouse brain (1) using Thymine DNA Glycosylase Antibody orb224021 with dilution at 1:1000.
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UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Thymine DNA Glycosylase Rabbit Polyclonal Antibody (orb224021)
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