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Description
Research Area
Images & Validation
−| Tested Applications | FC |
|---|---|
| Reactivity | Canine, Goat, Human, Monkey, Porcine, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | W8B2B10 |
| Immunogen | WERI-RB-1 retinoblastoma cell line |
| Target | TNAP |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 0.1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Separation of TNAP positive HeLa cells (red-filled) from TNAP negative HeLa cells (black-dashed) in flow cytometry analysis (surface staining) of HeLa cellular suspension stained using anti-TNAP (W8B2B10) PE antibody (concentration in sample 1.67 μg/ml).

Flow cytometry surface staining patterns of HeLa cells stained using anti-TNAP (W8B2B10) PE antibody (concentration in sample 1.67 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 1.67 μg/ml, same as TNAP PE concentration, right).
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TNAP Antibody (PE) (orb699837)
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