VIP Receptor 1/VIPR1 Rabbit Polyclonal Antibody

SKU: orb402544

Description

Anti-VIP Receptor 1/VIPR1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications	IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1.
Target	Vasoactive intestinal polypeptide receptor 1
Molecular Weight	52 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HVR1; II; PACAP R 2; PACAP R2; PACAP type II receptor; RDC1; VAPC1; VIP R 1; VIPR; VIPR1; VIRG; VPAC1; VPCAP1R

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VIP Receptor 1/VIPR1 Rabbit Polyclonal Antibody

Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human A431 cell lysates, Lane 2: human PANC-1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.

Quick Database Links

Gene Symbol

Vasoactive intestinal polypeptide receptor 1

UniProt

P32241

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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