Apoptozole

SKU: orb1304341

Description

Apoptozole is a small molecule apoptosis inducer that functions by inhibiting the ATPase activity of Hsc70 and Hsp70. Its application in research includes studying heat shock protein function, endoplasmic reticulum stress, and programmed cell death in both cellular and animal models of cancer.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	1054543-47-3
MW	625.56
Purity	99.75% (May vary between batches)
Formula	C₃₃H₂₅F₆N₃O₃
SMILES	COc1ccc(cc1)-c1nc(-c2cc(cc(c2)C(F)(F)F)C(F)(F)F)n(Cc2ccc(cc2)C(N)=O)c1-c1ccc(OC)cc1
Target	HSP,Apoptosis
Solubility	H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:24 mg/mL (38.37 mM);DMSO:93 mg/mL (148.67 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.28 mM)

Bioactivity

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Target IC50	HSP70:0.14 μM(Kd)\|HSC70:0.21 μM(Kd)
In Vivo	Apoptozole greatly retards tumor growth in mice xenografted with cancer cells without affecting mouse viability. The elimination half-life time (T1/2) of Az in blood is found to be significantly longer than that of Dox (8.04 versus 1.60 hr) and the time needed to reach a maximum concentration (Tmax) of Az is shorter than that of Dox (1.00 versus 4.00 hr). Read more...
In Vitro	Apoptozole induces caspase dependent apoptosis by blocking interaction of HSP70 with APAF-1, without affecting interactions of HSP70 with ASK1, JNK, BAX, and AIF. However, apoptozole may form aggregates under aqueous conditions that could interact with HSP70 proteins in a non-specific manner, potentially leading to false positives and inconsistent data. Read more...
Cell Research	Several cancer cell lines (A549, lung adenocarcinoma epithelial cells; HeLa, cervical cancer cells; MDA-MB-231, breast cancer cells; HepG2, liver cancer cells) are treated with various concentrations (0-15 μM) of Az or compound 7 as a negative control for 18 hr. Cell viabilities are then determined using an MTT assay.(Only for Reference) Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATPase, Apoptosis Activator VII, Apoptozole, Apoptosis, domain, Heat shock proteins, Inhibitor, Hsc70, HSC70, Hsp70, HSP70, HSP, inhibit

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Compound Identifiers

PubChem CID

24894064 ↗

Key Properties

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No computed properties available.

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Apoptozole (orb1304341)

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Purity:	>98% (HPLC)
CAS Number:	1054543-47-3
MW:	625.56
Formula:	C₃₃H₂₅F₆N₃O₃

Apoptozole

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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