ATG4B Antibody (HRP)

SKU: orb383939

Description

Rabbit polyclonal antibody against ATG4B conjugated to HRP.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

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Tested Applications	ICC, IF, WB
Dilution range	WB (1:1000), ICC/IF (1:100)
Reactivity	Human, Mouse
Application Notes	A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)
Target	ATG4B
Molecular Weight	44 kDa
Purification	Peptide Affinity Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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ATG4B, APG4B, AUTL1, KIAA0943, ATG4B_HUMAN, Autophagy-related protein 4 homolog B, Autophagin-1, Autophagin 1, Autophagy-related cysteine endopeptidase 1, AUT-like 1 cysteine endopeptidase, Cysteine protease ATG4B, APG4 autophagy 4 homolog B, ATG4 autophagy related 4 homolog B (S. cerevisiae), EC:3.4.22.-, hAPG4B, MGC1353

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.

Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band (s): Band appears at ~40 kDa.

Quick Database Links

Gene Symbol

ATG4B

UniProt

Q9Y4P1

NCBI Gene ID

23192

RefSeq (Protein):NP_037457.3

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_037457.3

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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