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B7-1 Fc Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 cells. The expected ED50 for this effect is 0.5-1.5 ug/ml. |
| Target | CD80 |
| Protein Sequence | VIHVTKEVKE VATLSCGHNV SVEELAQTRI YWQKEKKMVL TMMSGDMNIW PEYKNRTIFD ITNNLSIVIL ALRPSDEGTY ECVVLKYEKD AFKREHLAEV TLSVKADFPT PSISDFEIPT SNIRRIICST SGGFPEPHLS WLENGEELNA INTTVSQDPE TELYAVSSKL DFNMTTNHSF MCLIKYGHLR VNQTFNWNTT KQEHFPDNGG PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K |
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
Storage & Handling
−| Storage | The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human B7-1 Protein, mFc-His Tag [orb757420]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
E.coli
100 μg, 50 μg, 10 μgHuman B7-1 Protein, hFc Tag [orb689457]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μgMouse B7-1 Protein, hFc Tag [orb1290897]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgB7-1 (CD80) (NM_005191) Human Recombinant Protein [orb3036367]
> 80% as determined by SDS-PAGE and Coomassie blue staining
51 kDa
20 μg, 1 mgMouse CD80 protein [orb707166]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 10 μg, 50 μg

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UniProt Details
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Protocol Information
B7-1 Fc Recombinant Protein (orb1230423)
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