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Bcl-2 Antibody
SKU: orb749299
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | 100/D5 |
| Immunogen | Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot testing of human MCF7 cell lysate with Bcl-2 antibody (clone 100/D5).
SDS-PAGE analysis of purified, BSA-free Bcl-2 antibody (clone 100/D5) as confirmation of integrity and purity.
IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5). Note nuclear membrane & cytoplasmic staining.
Western blot testing of human samples using Bcl-2 antibody (100/D5).
FACS staining (intracellular) of Jurkat cells using Bcl-2 antibody (red) and isotype control (green).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
Bcl-2 Antibody (orb749299)
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