Recombinant Bcl6 Antibody

SKU: orb534365

Description

Bcl6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. [UniProt]

Research Area

Cancer, Epigenetics, Immunology

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bcl6 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	ARBC6-2R
Immunogen	Amino acids 256-389 were used as the immunogen for the recombinant Bcl6 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of FFPE human tonsil with recombinant Bcl6 antibody (clone ARBC6-2R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min.

Quick Database Links

UniProt

P41182

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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