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Bi-Phospho-ERK1/2(T202/Y204) Antibody
SKU: orb1931094
Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Infectious Disease & Virology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, WB |
|---|---|
| Dilution Range | WB - 1:1000, DB - 1:500 |
| Reactivity | Human |
| Predicted Reactivity | Drosophila, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 43136 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ERK1, PRKM3
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of extracts from Hela cells, untreated or treated with TPA (200nM), and NIH/3T3 cells, untreated or treated with PDGF (100ng/ml), using Phospho-ERK1-T202/Y204 Antibody (upper) or Tubulin (lower).
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Protocol Information
WB
Western Blot (IB, immunoblot)
DOT
Dot Blot
Bi-Phospho-ERK1/2(T202/Y204) Antibody (orb1931094)
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