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Blocking Buffer (2X) for Fluorescent Western Blotting
SKU: orb348639
Description
Images & Validation
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| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Purity | Blocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system. |
|---|---|
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store blocking buffer at 4° C prior to opening. DO NOT FREEZE. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Thimerosal is added as an antimicrobial agent.. See application note. |
| Concentration | 2 X |
| Hazard Information | Non-Toxic |
| Disclaimer | For research use only |
Alternative Names
−Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Western Blot Comparison of 1X and 2X orb348637 Buffer. Lane 1: reduced human NAG1 protein 0.1 µg. Lane 2: Prestained Molecular Weight Marker 5 µl. Lane 3: non-reduced human NAG1 protein 0.1 µg. Blocking Buffer: Left blot 1X, Right blot 2X of for 30 min at RT. Primary Antibody: rabbit anti-NAG1 biotin conjugated antibody (p/n orb345179) at 1 µg/ml overnight at 2-8°C. Secondary Antibody: goat anti-rabbit antibody HRP conjugated (p/n orb347654) at 1:70000 for 30 min at RT. Expect: ~14kDa for NAG1.
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Blocking Buffer (2X) for Fluorescent Western Blotting (orb348639)
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