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Blocking Buffer Sampler Kit
SKU: orb348636
Description
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| Tested Applications | ELISA, IF, IHC, Multiplex Assay, WB |
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| Application Notes |
Key Properties
−| Purity | Lyophilized normal goat serum was prepared from normal goat serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. BSA has Purity (%): 100% by Agarose Zone Electrophoresis Protease: < 0.005 units/mg by Enzymatic Assay. And no detectable IgG by Radial Immunodiffusion. Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell blocking buffer with stabilizer, and Fish gel concentrate were aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The products were tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and found to be negative for bacteria. |
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Storage & Handling
−| Storage | Do not freeze kit. Store kit at 4° C. Kit contains multiple components. See kit insert for complete instructions. |
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| Hazard Information | Non-Toxic |
| Disclaimer | For research use only |
Alternative Names
−Blocking Buffer Sampler Kit, Normal Goat Serum, NGS, BSA, Bovine Serum Albumin, Blocking Buffer for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer, TBS Fish Gel Solution
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Biorbyt produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n orb348581) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n orb348584) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n orb348587), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer, added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n orb348651) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Blocking Buffer Sampler Kit (orb348636)
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