Bovine Collagen Type III protein

SKU: orb750377

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, SDS-PAGE, WB
Application Notes	Bovine Collagen Type III can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

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Source	Bovine
Biological Origin	Bovine
Target	COL3A
Purity	Bovine Collagen Type III has been prepared from bovine placenta and is chromatographically and immunologically pure. Bovine Collagen Type III is free from other collagens, serum proteins and non-collagen extracellular matrix proteins. Bovine Collagen Type III reacts with anti-Collagen Type III. Reaction with anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.5 M Acetic Acid
Concentration	1 mg/ml
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Type III collagen protein, collagen 3 protein, bovine collagen protein

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SDS-PAGE Results of Bovine Collagen Type III. Lane 1: Bovine Collagen Type III Reduced (7.0 µg). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Bovine Collagen Type III Non-Reduced (7.0 µg). 7.5% Gel, Coomassie Stained. Observed MW: Type III trimer, dimer, monomer.

Quick Database Links

Gene Symbol

COL3A

UniProt

P04258

RefSeq:AAI23470.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAI23470.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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