Bovine Collagen Type IV protein

SKU: orb750378

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, SDS-PAGE, WB
Application Notes	Bovine Collagen Type IV can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Bovine Collagen Type IV is stable at 4°C as an undiluted liquid and should only be diluted to working range(s) just prior to use. Storing dilutions is not recommended.

Key Properties

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Source	Bovine
Biological Origin	Bovine
Target	COL4A1-COL4A4
Purity	Bovine Collagen Type IV has been prepared from Bovine Placenta and is chromatographically and immunologically pure. Bovine Collagen Type IV reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. Bovine Collagen Type IV is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Storing dilutions is not recommended.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid
Concentration	1.0 mg/ml
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Type IV collagen protein, collagen 4 protein, bovine collagen protein

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SDS-PAGE of Bovine Collagen Type IV. Lane 1: Bovine Collagen Type IV Reduced (5.0 µg). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Bovine Collagen Type IV Non-Reduced (5.0 µg). Observed MW: α1, α2, α3 bands. 4-20% Gel Coomassie Stained.

Quick Database Links

Gene Symbol

COL4A1-COL4A4

UniProt

Q28084

RefSeq:DAA32356.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	DAA32356.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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