Bovine Collagen Type V protein

SKU: orb750379

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, SDS-PAGE, WB
Application Notes	For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Key Properties

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Source	Bovine
Biological Origin	Bovine
Target	COL5A1/COL5A2
Purity	Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure. This product reacts with anti-Collagen Type V. Reaction with anti-Collagen I, II, III, IV or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid
Concentration	1.0 mg/mL
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Extracellular Matrix Protein protein

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SDS-PAGE of Bovine Collagen Type V. Lane 1: Bovine Collagen Type V Reduced (5.0 µg). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Bovine Collagen Type V Non-Reduced (5.0 µg). Predicted MW: > 95kDa. Observed MW: α1 and α2 bands. 4-20% Gel Coomassie Stained.

Quick Database Links

Gene Symbol

COL5A1/COL5A2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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