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Brefeldin A

SKU: orb1301107

Description

Brefeldin A is a macrolide antibiotic and potent ATPase inhibitor that induces tumor cell differentiation and apoptosis while also inhibiting autophagy. It is widely used in cancer research to study intracellular transport mechanisms in both cellular and animal models.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology, Pharmacology & Drug Discovery

Images & Validation

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Brefeldin A

Key Properties

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CAS Number20350-15-6
MW280.36
Purity99.90% (May vary between batches)
FormulaC16H24O4
SMILES[H][C@@]12C[C@H](O)C[C@@]1([H])[C@H](O)\C=C\C(=O)O[C@@H](C)CCC\C=C\2
TargetHSV,Autophagy,CRISPR/Cas9,Antibiotic,Antibacterial,ATPase,Mitophagy
SolubilityDMSO:128.75 mg/mL (459.23 mM);Ethanol:2.81 mg/mL (10.02 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (14.27 mM)

Bioactivity

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Target IC50
ATPase (HCT 116 cells):0.2 μM|HepG2 cells proliferation:239.1 nM|A549 cells proliferation:101.2 nM|HeLa cells proliferation:171.9 nM
In Vivo
In HF4.9 and HF28RA cells, Brefeldin A (25 ng/mL) completely inhibits cell growth. Similarly, in HF1A3 cells, Brefeldin A (75 ng/mL) fully inhibits cell growth.
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In Vitro
METHODS: Tumor cells HL60, K562 and HT-29 were treated with Brefeldin A (2 μM) for 72 h. DNA fragments were detected by DNA filter elution assay. RESULTS: Brefeldin A induced DNA fragmentation with different kinetics. intact DNA fragments were observed in HL60 cells within 15 h, whereas 48-72 h was required for K562 and HT-29 cells. METHODS: Human breast cancer cells MDA-MB-231 were treated with Brefeldin A (0.05-1 μg/mL) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: PARP cleavage, a hallmark event of cell death, could be detected in Brefeldin A-treated suspension MDA-MB-231 cells.
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Cell Research
HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)
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Storage & Handling

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Storagestore at low temperature,keep away from moisture,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Cas9, Brefeldin A, Cyanein, CRISPR/Cas9, CRISPR, Decumbin, BFA, Ascotoxin, ATPase (HCT 116), Autophagy, Antibiotic, inhibit, Mitochondrial Autophagy, Mitophagy, Inhibitor, Herpes simplex virus, HSV

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
133640201 ↗

Key Properties

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No computed properties available.

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Brefeldin A (orb1301107)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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2 mg
$ 80.00
1 ml x 10 mM (in DMSO)
$ 90.00
5 mg
$ 90.00
10 mg
$ 100.00
50 mg
$ 240.00
100 mg
$ 400.00
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