Recombinant Bromodeoxyuridine Antibody / BrdU

SKU: orb2644108

Description

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	All
Application Notes	Optimal dilution of the recombinant Bromodeoxyuridine antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rBRD.3
Immunogen	Bromodeoxyuridine conjugated to BSA was used as the immunogen for the BrdU antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Bromodeoxyuridine Antibody / BrdU

SDS-PAGE analysis of purified, BSA-free recombinant Bromodeoxyuridine antibody (clone rBRD.3) as confirmation of integrity and purity.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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