Canine CD3E & CD3D Heterodimer Protein, His Tag

SKU: orb1496139

Description

Canine CD3E & CD3D Heterodimer Protein, His Tag & Tag Free is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D) (Accession # P27597-1 (CD3E) & XP_536556.1 (CD3D)).

Images & Validation

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Application Notes

Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa (CD3E) under reducing (R) condition, and 36-45 kDa (CD3D) under non-reducing (NR) condition (SDS-PAGE).

Key Properties

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Expression System	HEK293
Target	CD3E & CD3D
Reactivity	Canine
Tag	C-10×His \| Native
Molecular Weight	16.6 kDa (CD3E) & 13.3 kDa (CD3D)
Expression Region	Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D)
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

CD3E & CD3D

UniProt

P27597

UniProt Details

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No UniProt data available

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