CCL8/MCP2 Antibody

SKU: orb1539111

Description

CCL8/MCP2 Antibody

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution Range	ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.25 - 0.5 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Reconstitute with sterile water to a concentration of 1 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-2 when used with biotinylated antigen affinity purified anti-human MCP-2 as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Immunogen	E.coli derived recombinant Human MCP-2 cprresponding to amino acids 24-99 of P80075.
Target	CCL8 / MCP2
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from PBS
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CCL8, C-C motif chemokine 8, HC14, MCP2, Monocyte chemotactic protein 2, Scya10, SCYA8, Small-inducible cytokine A8, MCP-2, Chemokine (C-C motif) ligand 8

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Quick Database Links

Gene Symbol

CCL8 / MCP2

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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