CD105 Antibody / Endoglin

SKU: orb639499

Description

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. CD105 has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. It is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Research Area

Cancer, Epigenetics

Images & Validation

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Tested Applications	WB
Dilution Range	Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Titering of the CD105 antibody may be required for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	ENG/1326
Immunogen	A partial recombinant protein corresponding to amino acids 74-251 of human CD105/Endoglin was used as the immunogen for the CD105 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human 1) HeLa, 2) A431 and 3) HL60 cell lysate with CD105 antibody (clone ENG/1326). Observed molecular weight: 70/90 kDa (monomer, unmodified/glycosylated); 140-180 kDa (dimer, unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free CD105 Antibody (clone ENG/1326). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P17813

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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