CD120b Hamster Monoclonal Antibody

SKU: orb2988685

Description

The CD120b Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against E. coli -expressed mouse Type II TNFR .Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution Range	IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Hamster
Clonality	Monoclonal
Isotype	Armenian Hamster IgG
Clone No.	TR75-54.7
Immunogen	E. coli -expressed mouse Type II TNFR
Target	TNFRSF1B
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TNFBR; TNFR2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept

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Quick Database Links

Gene Symbol

TNFRSF1B

UniProt

P25119

NCBI Gene ID

21938

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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