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CD16 Antibody

SKU: orb749494

Description

Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFACS, Functional Studies, IF
Dilution RangeFunctional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the CD16 antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2a, kappa
Clone No.HuNK2
ImmunogenHuman peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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CD16 Antibody

SDS-PAGE Analysis of Purified, BSA-Free CD16 Antibody (clone HuNK2). Confirmation of Integrity and Purity of the Antibody.

UniProt Details

No UniProt data available

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

CD16 Antibody (orb749494)

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Available Sizes

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20 μg
$ 370.00
100 μg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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