CD209/DC-SIGN Antibody

SKU: orb1538159

Description

Images & Validation

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Tested Applications	FC, IHC, IHC-P, IP
Dilution Range	Flo, IHC, IHC-P (20 µg/ml), IP
Reactivity	Human
Application Notes	Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml. Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Read more...

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2a,k
Clone No.	h209
Immunogen	Human CD209
Target	CD209 / DC-SIGN
Purification	Affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1

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Quick Database Links

Gene Symbol

CD209 / DC-SIGN

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Datasheet

Product Information

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Protocol Information

IHC

Immunohistochemistry

View Protocol

IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunoprecipitation