CD272 Rabbit Polyclonal Antibody

SKU: orb10311

This product can be custom made

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Description

CD272 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human B and T-lymphocyte attenuator (221-289/289aa)
Target	BTLA
Molecular Weight	28 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BTLA1; CD272; A630002H24; BTLA_HUMAN; BTLA; B- and T-lymphocyte-associated protein; BTLA_MOUSE; BTLA_RAT;

Quality Guarantee

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Sample: Lane 1: Rat Thymus tissue lysates, Lane 2: Rat Lung tissue lysates, Primary: Anti-CD272 (orb10311) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kDa, Observed band size: 55 kDa.

Tissue/cell: mouse lymphoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD272/BTLA Polyclonal Antibody, Unconjugated (orb10311) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

BTLA

UniProt

Q7Z6A9

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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