CD3 Antibody / CD3e

SKU: orb385668

Description

The CD3 complex mediates signal transduction. [UniProt]

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 5

Tested Applications	FACS, Functional Studies, IF
Dilution Range	Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG3, kappa
Clone No.	RIV9
Immunogen	Human peripheral lymphocytes were used as immunogen for the CD3 antibody. The epitope is known to be on the epsilon chain.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red).

Flow cytometry testing of human Jurkat cells with CD3 antibody (clone RIV9); Red = isotype control, Blue = CD3 antibody.

SDS-PAGE analysis of purified, BSA-free CD3 antibody (clone RIV9) as confirmation of integrity and purity.

Immunofluorescent staining of methanol-fixed human tonsil tissue cryosection with CF488A-CD3 antibody (clone RIV9, green) and DAPI nuclear stain (blue).

Flow cytometry testing of lymphocyte-gated human PBM cells with CF488A-CD3 antibody (clone RIV9); Gray = unstained, Green = CD3 antibody.

Quick Database Links

UniProt

P07766

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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