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CD3 (mouse specific) Rat Monoclonal Antibody
SKU: orb1294281
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, IHC |
|---|---|
| Dilution Range | Immunofluorescence 1:100-1:200, Immunohistochemistry (Frozen) 1:50-1:100, Flow Cytometry 1:50-1:100 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | Rat IgG2a |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Mouse CD3 |
| Target | CD3 (mouse specific) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate-buffered solution, pH 7.2, containing 0.025% ProClin 300 , 20% Glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294281 anti-CD3 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded mouse spleen tissue labeling CD3 with orb1294281 at 1/50.
CD3 antibody, orb1294281, Sample: C57BL/6 mouse splenocytes.
Quick Database Links
Gene Symbol
CD3 (mouse specific)
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Protocol Information
CD3 (mouse specific) Rat Monoclonal Antibody (orb1294281)
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