CD34 Antibody Cocktail

SKU: orb2639460

Description

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

Images & Validation

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Tested Applications	IHC-P
Dilution range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the CD34 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, lambda
Clone No.	QBEnd/10 + HPCA1/763
Immunogen	A detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10) and recombinant full-length human protein (HPCA1/763) were used as the immunogen for the CD34 antibody cocktail.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD34 antibody (QBEnd/10 + HPCA1/763)

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD34 antibody (QBEnd/10 + HPCA1/763)

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with CD34 antibody (QBEnd/10 + HPCA1/763)

IHC: Formalin-fixed, paraffin-embedded human placenta stained with CD34 antibody (QBEnd/10 + HPCA1/763)

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UniProt

P28906

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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