Recombinant CD34 Antibody

SKU: orb2642395

Description

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Images & Validation

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Tested Applications	FACS, IHC-P
Dilution range	Flow cytometry: 0.5-1ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	HPCA1/2598R
Immunogen	Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human angiosarcoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human breast carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human tonsil with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CD34 antibody (clone HPCA1/2598R) as confirmation of integrity and purity.

Flow cytometry testing of human Jurkat cells with CD34 antibody (clone HPCA1/2598R); Red = isotype control, Blue = CD34 antibody.

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UniProt

P28906

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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