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CD3E Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 4E2E8 |
| Immunogen | Purified recombinant fragment of CD3E expressed in E. Coli. |
| Target | CD3E |
| Molecular Weight | 23kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
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Western blot analysis of Jurkat cell lysate using CD3E antibody.

Flow cytometric analysis of Jurkat cells negative control (purple) using CD3E antibody (green)
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Protocol Information
CD3E Antibody (orb69252)
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