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CD3E antibody
SKU: orb389336
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | IF/ICC: 1:100-500, IHC-P: 1:100-500, WB: 1:100-600 |
| Reactivity | Guinea pig, Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CD3E. Please contact us for the exact immunogen sequence. The peptide is available as orb12965. |
| Target | CD3E |
| Molecular Weight | 23 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
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Immunohistochemical staining of paraffin embedded guinea pig rectum tissue using CD3E antibody (2.5 ug/ml)
Immunofluorescence image of guinea pig rectum tissue using CD3E antibody (2.5 ug/ml)
Immunohistochemical staining of rat lymph node tissue using CD3E antibody (2.5 ug/ml)
IHC-P image of guinea pig lung tissue using CD3E antibody (2.5 ug/ml)
Western blot analysis of rat lymph node (lane 1), rat spleen (lane 2), rat lung (lane 3), rat rectum (lane 4), human breast tumour (lane 5), Hela cells (lane 6) using CD3E antibody (1 ug/ml)
Immunofluorescence analysis of guinea pig rectum tissue using CD3E antibody (2.5 ug/ml)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
CD3E antibody (orb389336)
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