CD40 (phospho Thr254) rabbit pAb Antibody

SKU: orb770942

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Description

Rabbit polyclonal antibody to CD40 (phospho-Thr254)

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254. AA range:220-269
Molecular Weight	30
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-CD40 antibody, anti-TNFRSF5 antibody, anti-Tumor necrosis factor receptor superfamily member 5 antibody, anti-B-cell surface antigen CD40 antibody, anti-Bp50 antibody, anti-CD40L receptor antibody, anti-CDw40 antibody, anti-CD antigen CD40 antibody

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CD40 (phospho Thr254) rabbit pAb Antibody

Western Blot analysis of COLO cells using Phospho-CD40 (T254) Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using TNFRSF5 (Phospho-Thr254) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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