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CD40 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40, His Tag at 5 ug/mL (100 uL/well) can bind Mouse CD40 Ligand, Fc Tag with a linear range of 1-16 ng/mL. |
| Target | CD40 |
| Tag | His Tag |
| Molecular Weight | 20.8 kDa |
| Protein Sequence | Val 24 - Arg 193 |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CD40L [orb2995248]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994033]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.1 KDa. Observed: 19-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2994532]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD40L [orb2993921]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human CD40L [orb2993734]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
20 μg, 100 μg

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Mouse CD40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
CD40 Recombinant Protein (orb1227695)
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