CD45RA Antibody (Leukocyte marker)

SKU: orb385828

Description

CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

Images & Validation

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Tested Applications	FACS, IF, IHC, WB
Dilution range	Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2 ug/ml,Immunohistology (FFPE): 0.5-1.0ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Titering of the anti-CD45RA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	CDLA45RA-1
Immunogen	Recombinant human protein was used as immunogen for this anti-CD45RA antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human tonsil and anti-CD45RA antibody (clone CDLA45RA-1). HIER: boil sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1); Red = isotype control, Blue = CD45RA antibody.

Western blot testing of human spleen lysate with CD45RA antibody (clone CDLA45RA-1). Expected molecular weight: 147-220 kDa depending on glycosylation level.

Immunofluorescence staining of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1, green) and Reddot nuclear stain (red).

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UniProt

P08575

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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