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CD66a/c/e Recombinant Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC |
|---|---|
| Dilution Range | ICC/IF=1:100-500, Flow-Cyt=1:50-100 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2b/Kappa |
| Clone No. | C5C6 |
| Target | CEACAM1 |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Cell line: HT-29, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173085.

Cell line: HT-29, Fixative: Unfixed, Permeabilization: None, Primary Ab Dilution: 1:100, Secondary Ab: Goat anti Mouse IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Mouse monoclonal IgG2b (Blue line). Comment: Line red is the positive signal for orb1173085.
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Protocol Information
CD66a/c/e Recombinant Mouse Monoclonal Antibody (orb1173085)
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