CD73, Human, mAb 4G4 Antibody

SKU: orb1108878

This product can be custom made

Description

Mouse monoclonal antibody to CD73

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	FC, Functional Studies, IF, IHC-Fr, IP, WB
Dilution Range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Human
Application Notes	F: Tissue sections were fixed in acetone.- As positive control anti-CD31 was used and as negative control an isotype antibody (Ref.5). FC: Antibody 4G4 stains the extracellular domain of CD73. As positive control anti-CD3- was used and as negative control an irrelevant antibody (Ref.1). FS: Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer (Ref.1). Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes (Ref.5) and reduces enzyme activity (Ref.2). IF: HUVEC cells were seeded on gelatin-coated coverslips and stained with antibody (Ref.5). Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1
Immunogen	Inflamed synovial stroma from rheumatoid arthritis patients
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
Buffer/Preservatives	Quantities >500 µg will be sent without preservatives and are carrier-free.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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5'-nucleotidase, ecto (CD73) Gene name: NT5E

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Immunoprecipitation