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ODF2 Antibody
SKU: orb345606
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, WB: 1:200 - 1:2,000 |
| Reactivity | Human, Monkey, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1. |
| Target | ODF2 |
| Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Cenexin-1 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with Cenexin-1from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Cenexin-1 pS796 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2, Cenexin1 variant 1 antibody, KKT 4 antibody, ODF2, Cenexin 1, Cenexin-1, Outer dense fiber protein 2
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ELISA of Rabbit Anti-Cenexin-1 pS796 Antibody. Antigen: BSA conjugates of Cenexin-1 pS796 (purple) and S796 (blue). Coating amount: 0.1 µg per well. Primary antibody: Cenexin-1 pS796 at 5 µg/ml. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8500. Substrate: TMB.
Western Blot of Rabbit Anti-Cenexin-1 pS796. Lane 1: MW. Lane 2: Human Semen Lysate. Lane 3: MCF7 WCL (p/n orb348664). Lane 4: Molt 4 WCL (p/n orb348696). Lane 5: HeLa WCL (p/n orb348668). Load: 10 µg per lane. Blocking: (p/n orb348644). Primary Antibody: Anti-Cenexin-1 pS796 at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) 1:40000 diluted in (p/n orb348644) for 30 minutes at RT. Expect: 93kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ODF2 Antibody (orb345606)
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