Chicken anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse IgG

SKU: orb700358

Description

Gallus polyclonal antibody against Gallus anti-Rabbit IgG (H&L) conjugated to HRP

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	The optimal working dilution should be determined by the investigator
Reactivity	Rabbit
Application Notes	This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.

Key Properties

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Host	Gallus
Clonality	Polyclonal
Immunogen	Purified Rabbit IgG
Purity	Immunogen affinity purified chicken IgY.
Purification	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Conjugation	HRP

Storage & Handling

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Storage	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/Appearance	Lyophilized
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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