CK-7 Recombinant Rabbit Monoclonal Antibody

SKU: orb1151996

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:50-200, IF=1:200-1000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	C2G6
Immunogen	KLH conjugated synthetic peptide derived from human CK-7
Target	KRT7
Molecular Weight	54 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Cytokeratin 7; Cytokeratin-7; CK 7; CK-7; K7; Keratin 7; Keratin, type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN.

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CK-7 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: HeLa, MCF-7, A549, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 51 kDa, Observed MW: 50 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151996.

Tissue: Human breast cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151996.

Tissue: Human ovarian carcinoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151996.

Quick Database Links

Gene Symbol

KRT7

UniProt

P08729

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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