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IFT74 Antibody

SKU: orb1247666

Description

IFT74 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityRat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.2-0.6µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KTIVDALHSTSGN
TargetIFT74
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

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IFT74 Antibody

orb1247666 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

IFT74 Antibody

orb1247666 (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IFT74 Antibody

A) orb1247666 (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IFT74 Antibody (orb1247666)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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