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COL17A1 Antibody (Center)
SKU: orb1928110
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB: 1:2000, FC: 1:25 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB22700 |
| Target | This COL17A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1. |
| Molecular Weight | 150419 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, 120 kDa linear IgA disease antigen, 120 kDa linear IgA dermatosis antigen, Linear IgA disease antigen 1, LAD-1, 97 kDa linear IgA disease antigen, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa LAD antigen, 97-LAD, Linear IgA bullous disease antigen of 97 kDa, LABD97, COL17A1, BP180, BPAG2
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All lanes: Anti-COL17A1 Antibody (Center) at 1:2000 dilution. Lane 1: A2058 whole cell lysate. Lane 2: A375 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 150 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A431 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Quick Database Links
Gene Symbol
This COL17A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1.
UniProt
RefSeq (Protein):NP_000485.3
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000485.3 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
COL17A1 Antibody (Center) (orb1928110)
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