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COL6A1 Antibody (N-term)
SKU: orb1929961
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB19576 |
| Target | This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human COL6A1. |
| Molecular Weight | 108529 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Collagen alpha-1(VI) chain, COL6A1
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COL6A1 Antibody (N-term) [orb2996523]
FC, IHC-P, WB
Mouse
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Flow cytometric analysis of 293 cells using COL6A1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of COL6A1 antibody (N-term) in A2058 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified Pab.
Quick Database Links
Gene Symbol
This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human COL6A1.
UniProt
RefSeq (Protein):NP_001839.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001839.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
COL6A1 Antibody (N-term) (orb1929961)
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