Cyclo(his-pro)

SKU: orb1220309

Description

Cyclo(his-pro) is an endogenous cyclic dipeptide that exerts oxidative damage protection by selectively activating the transcription factor Nrf2 signalling pathway.

Images & Validation

−

Key Properties

−

CAS Number	53109-32-3
MW	234.25
Purity	>98% (HPLC)
Formula	C₁₁H₁₄N₄O₂
SMILES	O=C1NC(Cc2cnc[nH]2)C(=O)N2CCCC12
Target	Endogenous Metabolite
Solubility	H2O:30 mg/mL (128.07 mM)

Bioactivity

−

In Vivo

Cyclo(his-pro) (Cyclo(histidyl-proline); 1.8 mg/ear; topical application on the right ear; 30 min prior to TPA) reduces TPA-induced ear oedema confirming that it can exert anti-inflammatory effect. Cyclo(his-pro) exerts In vivo anti-inflammatory effects in the central nervous system by down-regulating hepatic and cerebral TNFα expression thereby counteracting LPS-induced gliosis. Moreover, by up-regulating Bip, Cyclo(his-pro) increases the ER stress sensitivity andtriggers the unfolded protein response to alleviate the ER stress. Animal model: Sixty two/three month-old male C57BL/6 mice (25-30 g). Dosage: 1.8 mg/ear. Administration: Topical application on the right ear; 30 min prior to TPA. Result: Reduced TPA-induced ear oedema.

In Vitro

cyclo(his-pro) (Cyclo(histidyl-proline); 50 μM; 1-48 hours) increases the nuclear level of Nrf2 and inhibits NF-κB nuclear translocation. Cyclo(His-Pro) alone has no effect on nuclear translocation of these transcription factors. cyclo(his-pro) (50 μM; prior to PQ exposure for 48 hours) abolishes protein nitration that followed paraquat (PQ) exposure and lessenes its functional consequences, as shown by decrease in cell apoptosis, detected by caspase 3 activity and by cytochrome c release. Cyclo(his-pro) inhibits NF-κB nuclear accumulation induced by paraquat in rat pheochromocytoma PC12 cells via the Nrf2/heme oxygenase-1 pathway. Western blot analysis. Cell line: PC12 cells. Concentration: 50 μM. Incubation time: 1, 2, 4, 8, 24, 48 hours. Result: Increased the nuclear level of Nrf2 and inhibited NF-κB nuclear translocation.

Storage & Handling

−

Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol

Endogenous Metabolite

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

Cyclo(his-pro) (orb1220309)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

Purity:	99.87% (May vary between batches)
MW:	234.25

CAS Number:	936749-56-3
MW:	348.282
Formula:	C₁₃H₁₅F₃N₄O₄

Cyclo(his-pro)

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Similar Products

Cyclo(his-pro) [orb1299754]

Cyclo(his-pro) TFA [orb3177695]

Quality Guarantee

Quick Database Links

Documents Download

Request a Document

Protocol Information

Cyclo(his-pro) (orb1220309)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors