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DCTN2 Antibody
SKU: orb1258223
Featured
Description
Research Area
Cell Biology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1). |
| Target | DCTN2 |
| Molecular Weight | Observed: 44kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, DCTN-50, p50 dynamitin, DCTN2, DCTN50
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Western blot analysis of extracts of MCF-7 cells, using DCTN2 antibody (orb1258223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody (orb1258223) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DCTN2 antibody (orb1258223) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using DCTN2 antibody (orb1258223) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DCTN2 antibody (orb1258223). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
DCTN2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
DCTN2 Antibody (orb1258223)
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