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DEFA1/3 Antibody

SKU: orb1249192

Description

DEFA1/3 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). . An additional band of unknown identity was also consistently observed at 20kDa, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/mL. . Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3-6µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DESLAPKHPGSRKN
TargetDEFA1/3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DEFA1, defensin, alpha 1, myeloid-related sequence , HGNC:2761, DEF1, DEFA2, HNP-1, HP-1, MRS , defensin, alpha 1, defensin, alpha 2, myeloid-related sequence, DEFA3, defensin, alpha 3, neutrophil-specific, DEF3, HNP-3, HNP3, HP-3, defensin 3, neutrophil-specific, defensin, alpha 3, neutrophil peptide 3

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DEFA1/3 Antibody

orb1249192 (2 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer).Detected by chemiluminescence.

DEFA1/3 Antibody

orb1249192 (3.8 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

DEFA1/3 Antibody (orb1249192)

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0.1 mg
$ 920.00
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