Dog Gastrin (GT) ELISA Kit

SKU: orb779822

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastrin(GT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastrin(GT).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog GT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Canine
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Competitive
Assay Time	2.5h
Range	15.63-1000 pg/mL
Sensitivity	4.9 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GAST ELISA Kit, GAS ELISA Kit, Gastrin component I ELISA Kit, Big gastrin ELISA Kit, Gastrin component II ELISA Kit, Gastrin component III ELISA Kit

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UniProt

P01353

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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